culture conditions murine hepatocyte cell line aml12  (ATCC)

Journal: Cancer research

Article Title: Obesity/type 2 diabetes-associated liver tumors are sensitive to Cyclin D1 deficiency

doi: 10.1158/0008-5472.CAN-20-0106

Figure Lengend Snippet: A. Metformin prevents insulin-induced Cyclin D1 protein levels in hepatocytes. Overnight serum starved AML12 murine hepatocytes were treated with control, metformin (10 mM), insulin (200 nM) or both for 4 h, followed by immunoblotting to detect Cyclin D1 expression. B. Design and metabolic phenotypes of the metformin treatment experiment. Metformin was given at 50 mg/kg for 27 weeks. Endpoint blood insulin level and liver histology of the db/db mice were shown. Scale bar represents 500 μm. C. Metformin treatment prevents the induction of Cyclin D1 in type 2 diabetic liver as detected by immunoblotting. D. Metformin treatment protects mice from DEN-induced liver cancer in diabetic mice (water, n=5; metformin, n=4).

Article Snippet: Tissue Culture AML12 murine hepatocyte cell line was obtained from ATCC, and maintained in DMEM/F12 (Sigma-Aldrich) with 10% FBS, 10 μg/ml insulin, 5.5 μg/ml transferrin, 5 ng/ml selenium, 40 ng/ml dexamethasone, 100 U/ml penicillin, and 100 mg/ml streptomycin.

Techniques: Control, Western Blot, Expressing